CP43' | IsiA homolog of plant CP43 positive control/quantitation standard
AS10 111S | recombinant protein standard for quantitation
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DATA SHEET IN PDF| Datasheet | Product citations | Protocols | Add review |
Product Information
Standard curve: 3 loads are recommended (2.5 and 10 μl).
For most applications a sample load of 0.2μg of chlorophyll will give a IsiA signal in this range.
Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.
This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
27 kDa (slightly larger than native protein due to His-tag)
Reactivity
Application examples
Additional information
The IsiA protein standard can be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in theIsiA protein.
Quantitative western blot: detailed method description, video tutorial
Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.15 pmoles/µl
Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.
This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.
Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Background
This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Product citations
Ryan-Keogh et al. (2012). Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo. J. of Phycology, 1:145-154.
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