Donkey anti-Rabbit IgG (H&L), DyLight® 633 conjugated,min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG

Product no: AS12 2034

AS12 2034 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG

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  • Product Info
  • Immunogen:

    Purified Rabbit IgG

    Host: Donkey
    Clonality: Polyclonal
    Purity: Immunogen affinity purified donkey IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Recommended dilution: 1 : 20-1 : 2000 for most applications
  • Additional Information
  • Additional information:

    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

    Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin rabbit serum proteinsIgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat.
    Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.

    Additional information (application):


    immunolocalization using Agrisera donkey anti-rabbit secondary antibody

    Donkey anti-rabbit conjugated to DY Light 633 in the absence of primary antibody show very little background label (right image) and binds to primary antibodies (anti-Arf1 and anti-Sec21p; two left images). The top images show only the antibody label in red and bottom images show overlay of antibody label in red with DAPI in blue and DIC/Nomarski image in grey. The material has been prepared using whole mount immunolabelling procedure of 5 day old Arabidopsis thaliana seedlings (Fischer, U. et al. (2006). Curr. Biol. 16, 2143–2149). Antibody dilutions: rabbit anti-sec21p (Agrisera, AS08 327) 1:1000, rabbit anti-Arf1 (Agrisera, AS08 325) 1:1000 and Agrisera Donkey anti-rabbit DY Light 633 1:100. DNA was stained with DAPI.

    Courtesy: Dr. Anna Gustavsson and Prof. Markus Grebe, Umeå Plant Science Centre

  • Background
  • Background:

    Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight®633 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight® 633, which binds to rabbit IgG (H&L) in immunological assays.

    DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are affinity purified using solid phase rabbit IgG (H&L).

    DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

  • Protocols
  • Antibody protocols
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