HSP90 | Heat shock protein 90

AS05 063 | Clonality: Polyclonal | Host: Rabbit | Reactivity: salmon, Human


product information
Background		HSP90 is a ubiquitous chaperone involved in numerous cellular processes. Members of this family of proteins play important roles in allowing a selected group of intracellular signaling molecules reach and maintain functionally active conformations.Alternative names: for hsp90 alpha: HSP 86, renal carcinoma antigen NY-REN-38, for hsp90 beta: hsp84
Immunogen		KLH-conjugated synthetic peptide chosen from a highly conserved region of hsp90 found in both the alpha P07900 and beta P08238 form of the protein. The target peptide is perfectly conserved in animals.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		100 µl
Reconstitution		For reconstitution add 100 µl of sterile water
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS05 061 \| anti-salmonid HSP70 AS05 061A \| anti-salmonid HSP70, suitable for immunoprecipitation AS05 062 \| anti-salmonid HSC70 AS05 083A \| anti-HSP70/HSC70 global antibody, affinity purified Secondary antibodies
Additional information

application information
Recommended dilution		1 : 5 000 (WB)
Expected \| apparent MW		84-86 kDa
Confirmed reactivity		Human, Salmon
Predicted reactivity		Fishes, Hen, Mammals
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		in salmonid fish a cross-reactive band at approximately 40 kDa is observed; antibody will also detect a human recombinant HSP90 protein Antibody is reacting strongly with recombinant HSP90 from HELA cells.
Selected references		Kelly et al. (2017). Acclimation capacity of the cardiac HSP70 and HSP90 response to thermal stress in lake trout (Salvelinus namaycush), a stenothermal ice-age relict. Comp Biochem Physiol B Biochem Mol Biol. 2017 Dec 10. pii: S1096-4959(17)30191-4. doi: 10.1016/j.cbpb.2017.12.002. Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015. Liu et al. (2014). Spermidine Enhances Waterlogging Tolerance via Regulation of Antioxidant Defence, Heat Shock Protein Expression and Plasma Membrane H+-ATPase Activity in Zea mays. J. Agronomy and Crop Science, Article first published online: 1 APR 2014, DOI: 10.1111/jac.12058. Chandra et al. (2012). Sustained high temperature increases the vitellogenin response to 17 alpha-ethynylestradiol in mummichog (Fundulus heteroclitus). Aquatic toxicology.

application example - Western blot:

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