HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70 (serum)
AS05 083 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global abtibody] for HSP70 and HSC70 in fish, mammals and fungi
Benefits of using this antibody

Data sheet | Product citations | Protocols | Add review |
Product Information
KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142
70 kDa
Reactivity
Application examples
Application example
10 µg of total protein from (1) killi fish muscle, (2) bovine muscle, (3) chicken muscle, (4) rat liver, extracted with Protein Extration Buffer, PEB (AS08 300) and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked in 5 % non-fat milk for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 5000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (Agrisera anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent, according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 5 min.
Additional information
For detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371.
Background
Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1, HSP70-1/HSP70-2, Heat shock 70 kDa protein 8
Product citations
Bessemer et al. (2014). Cardiorespiratory toxicity of environmentally relevant zinc oxide nanoparticles in the freshwater fish Catostomus commersonii. Nanotoxicology. 2014 Nov 27:1-10.
Gorovits et al. (2013). Recruitment of the host plant heat shock protein 70 by tomato yellow leaf curl virus coat protein is required for virus infection. PLoS Once, July 23;8(7).
Related products: HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70 (serum)
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