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AGO set
Tag Antibody Set 

HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70, Affinity purified

AS05 083A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global abtibody] for HSP70 and HSC70 in fish, mammals and fungi

HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70, Affinity purified in the group Antibodies for Human/Animal  / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 083A)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142 

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (IP), 1 : 1000-1: 5000 (WB)
Expected | apparent MW

70 kDa

Reactivity

Confirmed reactivity Fish, mammals, fungi: Antrodia infirma, A. sinuosa, A. xantha, Gloeophyllum protractum, Gloeophyllum sepiarium, G. carbonarium, Junghunia luteoalba, Mustelus canis, Oligoporus sericiomollis, Phlebia cornea, Squalus acanthias
Predicted reactivity Bovine, Drosophila melanogaster, Hen, Mouse, Rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

For detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371

This antibody is not suitable for work with samples from higher plants

Related products

Related products AS05 083 | Anti-HSP70/HSC70 | Heat shock protein 70/Heat shock cognate protein 70 (serum), rabbit antibodies

Secondary antibodies

Background

Background

Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1,   HSP70-1/HSP70-2,  Heat shock 70 kDa protein 8

Product citations

Selected references Bockus (2016). A Study of the Regulatory and Environmental Factors Affecting Trimethylamine Oxide Accumulation in Marine Organisms. Open Access Dissertations. Paper 513.
Tunnah et al (2016). Physiological responses to hypersalinity correspond to nursery ground usage in two inshore shark species (Mustelus antarcticus and Galeorhinus galeus). J Exp Biol. 2016 Jul 1;219(Pt 13):2028-38. doi: 10.1242/jeb.139964. Epub 2016 May 9.
French et al. (2015). High survivorship after catch-and-release fishing suggests physiological resilience in the endothermic shortfin mako shark (Isurus oxyrinchus). Conservation Physiology, Vol 3, Issue 1, 10.1093/conphys/cov044

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