Pectic polysaccharide, alpha-1,5-arabinan (monoclonal, clone LM13)
AS18 4200-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
Pectic polysaccharide, alpha-1,5-arabinan, This antibody was isolated from a high throughput screen of many antibodies generated by immunization with a pectic fraction,
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgM
Purity
Cell culture supernatant.
Format
Liquid
Quantity
1 ml
Storage
Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunoflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Contains 0.05% Sodium Azide.
Antibody recognition of arabinans increases with arabinofuranosidase action.
Binds to a specific subset of pectic arabinans, and to longer stretches of 1,5-linked arabinosyl residues that are likely to be more abundant in unbranched arabinans.
Antibody recognition of arabinans increases with arabinofuranosidase action.
Binds to a specific subset of pectic arabinans, and to longer stretches of 1,5-linked arabinosyl residues that are likely to be more abundant in unbranched arabinans.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.
Product citations
Selected references
Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.
Moller et al. (2008). High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray. inding profiles Glycoconj J. 2008 Jan;25(1):37-48. doi: 10.1007/s10719-007-9059-7.
Moller et al. (2008). High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray. inding profiles Glycoconj J. 2008 Jan;25(1):37-48. doi: 10.1007/s10719-007-9059-7.
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