Can the same protocol can be applied to all antibodies? No, this is NOT the case. Each protein-antibody pair may require slightly modified binding and detection conditions. The variations may for example be a membrane with another pore size and physical properties (nitrocellulose or PVDF) as well as different blockers, incubation times and primary antibody dilutions. Some antibodies may work better in TBS-T buffer, others with PBS-T. These issues are disussed in Agrisera Western blot guide, which we recommend you to keep in your lab! | Western blot guide Get your free copy of the Agrisera Western Blot Guide here! |
Latest
What parameters should be consider when transferring HMW proteins?2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20 Are directly conjugated primary antibodies always the best choice?
2023-12-28 How to obtain sharper protein bands in Western blot?
2023-12-20 Are monoclonal antibodies better, compared to polyclonal antibodies?
2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18 Antibody suddenly does not work any longer, what can be the reason?
2023-07-11 Can an antibody made to a full-length protein distinguish between 2 isoforms?
2023-05-10
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