Is the antibody always to blame?

In the example blot to the right, the same antibody was used (Anti-BAK1, AS12 1858) but the proteins were extracted with two different buffers.
Minor adjustments in the buffer components resulted in the improved detection of the target protein - from no band detected at all as seen in the left panel to a band of correct MW visible in the right panel.

If you are interested to learn more how to optimize your western blot, you are welcome to contact us or request a copy of the Agrisera Western Blot Guide.

Western blot using anti-BAK1 antibodies

Technical blog

Technical blog

Latest

What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20 Are directly conjugated primary antibodies always the best choice?
2023-12-28 How to obtain sharper protein bands in Western blot?
2023-12-20 Are monoclonal antibodies better, compared to polyclonal antibodies?
2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18 Antibody suddenly does not work any longer, what can be the reason?
2023-07-11 Can an antibody made to a full-length protein distinguish between 2 isoforms?
2023-05-10

Login / RetailerClose

Forgot password?