In the example blot to the right, the same antibody was used (Anti-BAK1, AS12 1858) but the proteins were extracted with two different buffers.
Minor adjustments in the buffer components resulted in the improved detection of the target protein - from no band detected at all as seen in the left panel to a band of correct MW visible in the right panel. 

If you are interested to learn more how to optimize your western blot, you are welcome to contact us or request a copy of the Agrisera Western Blot Guide.



 Western blot using anti-BAK1 antibodies


 Technical blog

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