| Western Blotting Troubleshooting Tips from experienced users of this technique were published in August 2021 on Biocompare. Interviewees for the article were: Joanna Porankiewicz-Asplund, Ph.D., Technical Support Manager at Agrisera AB Tobias Polifke, Co-founder and Managing Director at CANDOR Bioscience GmbH Rebecca Northeast, Technical Specialist at Proteintech Group. Anne Sloan, Ph.D., Technical Support Scientist at Cell Sciences Learn some tips you may not know about! |  Agrisera Western Blot Resource (protocols, videos and more) |
Technical tips
Latest
Can methanol in transfer buffer be replaced by a less toxic alternative?2025-05-12 Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?
2025-04-29 How to check if protein transfer from a gel to a membrane was efficient
2025-04-15 How to detect membrane proteins on Western blot?
2025-03-27 Rinse Right: The Hidden Power of Washing in Western Blots
2025-02-11 Can one Western blot protocol fit all antibodies?
2025-02-07 How to reconstitute lyophilized antibodies?
2025-01-17 What information to look for before purchasing an antibody?
2024-12-30 Membrane activation before protein transfer: which one to use, methanol or ethanol?
2024-12-23 Comparing antibodies between each other, why does it not work?
2024-11-25
Archive
- May - 2025
- April - 2025
- March - 2025
- February - 2025
- January - 2025
- December - 2024
- November - 2024
- September - 2024
- July - 2024
- June - 2024
- May - 2024
- March - 2024
- February - 2024
- December - 2023
- November - 2023
- September - 2023
- July - 2023
- May - 2023
- March - 2023
- January - 2023
- December - 2022
- November - 2022
- October - 2022
- September - 2022
- August - 2022
- June - 2022
- May - 2022
- March - 2022
- February - 2022
- January - 2022
- November - 2021
- October - 2021
- August - 2021
- June - 2021
- May - 2021
- April - 2021
- March - 2021
- February - 2021
- January - 2021
- December - 2020
- November - 2020
- October - 2020
- September - 2020
- August - 2020
- July - 2020
- June - 2020
- May - 2020
- April - 2020
- March - 2020
- January - 2020
- November - 2019
- October - 2019
- March - 2019
- April - 2017
- February - 2017
- May - 2016
- February - 2014
- September - 2013
- December - 2010