| Western Blotting Troubleshooting Tips from experienced users of this technique were published in August 2021 on Biocompare. Interviewees for the article were: Joanna Porankiewicz-Asplund, Ph.D., Technical Support Manager at Agrisera AB Tobias Polifke, Co-founder and Managing Director at CANDOR Bioscience GmbH Rebecca Northeast, Technical Specialist at Proteintech Group. Anne Sloan, Ph.D., Technical Support Scientist at Cell Sciences Learn some tips you may not know about! |  Agrisera Western Blot Resource (protocols, videos and more) |
Technical tips
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Calclulated and aparent molecular weight of detected protein is different, why?2025-10-09 How to chose right loading control for Western blot?
2025-10-06 Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-03 Is an antibody going to work in a technique I am planning to use it in?
2025-09-30 Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample
2025-08-20 Can aggregated antibody be still used?
2025-08-15 Blot Once, Probe Twice - when such approach can be beneficial?
2025-07-31 Why should glycerol not be added, to stabilize antibodies in serum?
2025-06-27 Can methanol in transfer buffer be replaced by a less toxic alternative?
2025-05-12 Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?
2025-04-29
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