DYKDDDDK (binds to Sigma FLAGŪ) (mouse antibody,monoclonal, Clone 1E6)
AS15 3036 | Clonality: Monoclonal | Host: Mouse | Reactivity: DYKDDDDK-tag
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Product Information
Immunogen
KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®)
Host
Mouse
Clonality
Monoclonal
Purity
Protein G purified
Format
Liquid in PBS, pH 7.4, with 0.05% sodium azide
Quantity
100 µg
Storage
Store at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 100 (WB) 1 : 20 (IP), 1 : 20 (IHC)
Reactivity
Confirmed reactivity
DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in mammalian cells
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Background
Background
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product citations
Selected references
Durall et al. (2020). Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels. 2020 Jan 28;13:16. doi: 10.1186/s13068-020-1653-y.
Wardhan et al. (2017). Chickpea transcription factor CaTLP1 interacts with protein kinases, modulates ROS accumulation and promotes ABA-mediated stomatal closure. Sci Rep. 2016 Dec 9;6:38121. doi: 10.1038/srep38121.
Wardhan et al. (2017). Chickpea transcription factor CaTLP1 interacts with protein kinases, modulates ROS accumulation and promotes ABA-mediated stomatal closure. Sci Rep. 2016 Dec 9;6:38121. doi: 10.1038/srep38121.
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