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AGO set
Tag Antibody Set 

PsbA | D1 protein of PSII, C-terminal, HRP-conjugated (40 ĩg)

AS05 084A-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

PsbA | D1 protein of PSII, C-terminal, HRP-conjugated (40 ĩg) in the group Antibodies for Plant/Algal  / Global Antibodies at Agrisera AB (Antibodies for research) (AS05 084A-HRP)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

Host Rabbit
Clonality Polyclonal
Purity Affinity purified
Format Liquid, conjugated to HRP
Quantity 40 µg
Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Tested applications Western blot (WB)
Recommended dilution 1 : 15 000 (WB)
Expected | apparent MW

38 | 28-30 kDa

Reactivity

Confirmed reactivity Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp., Ditylum brightwellii,  Glycine max, Hordeum vulgare, Lindernia sp., Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana, Panicum miliaceum, Panax ginseng, Panicum maximum, Pinus strobus, Physcomitrella patens, Synechococcus sp. PCC 7942, Paulinella chromatophora (amoeba), Prochlorococcus sp. (surface and deep water ecotype), Spirodela polyrhiza,  Symbiodinium sp., Zea mays
Predicted reactivity

Algae (brown and red), Dictos, Conifers, Cyanobacteria, Medicago sativa, Pisum sativum, Triticum aestivum, cellular [compartment marker] of thylakoid membrane.

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.

Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.

Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.

This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.

The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.

Related products

Related products

AS01 016S | PsbA protein standard for a quantitative western blot
AS05 084PRE | PsbA | D1 protein of PSII, C-terminal , pre-immune serum
AS08 084A | Anti-PsbA | D1 protein of PSII, C-terminal, rabbit antibodies
AS11 1786  | Anti-PsbA N-terminal, rabbit antibodies
AS10 704 | Anti-PsbA | D1 protein of PSII, DE-loop, rabbit antibodies
AS13 2669 | Anti-PsbA | D1 protein of PSII, phosphorylated, rabbit antibodies

Plant and algal protein extraction buffer

Background

Background The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.

Product citations

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