PsbP-like protein (sll1418) | cyanobacterial

Product no: AS10 682

AS10 682  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: cyanobacteria

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from PsbP -like protein of Synechocystis sp. PCC 6803, UniProt: P73952

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW: 20,7 | 17 kDa
  • Reactivity
  • Confirmed reactivity: Synechocystis sp. PCC 6803
    Predicted reactivity:

    Anabaena variabilis, Arthrospira maxima, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes, Ostreococcus lucimarinus, Trichodesmium erythraeum,


    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot detection using anti-PsbP-like antibody

    0.25 µg of chlorophyl a/lane from Synechocystis sp. PCC 6802 wilde type and PsbP-like deletion mutants were separated on 12.5 % SDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbP-like antibody (AS10 682, 1:2 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL detection reagent according the manufacturers instructions. Exposure time was 15 seconds.

    Courtesy Dr. Sveshnikov

  • Background
  • Background:

    PsbP-like protein (sll1418) is a cyanobacterial homologue of plant PsbP-like protein. It is localized in the thylakoid membrane and associated with photosystem II. Synonymes: Sll1418 protein

  • Product Citations
  • Selected references: Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
    Sveshnikov et al. (2007) The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II, Photosynth. Res. 93, 101-109.
    Ishikawa et al. . (2005) Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803, Photosynth. Res. 84, 257-262.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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