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PsbP-like protein (sll1418) | cyanobacterial

AS10 682  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: cyanobacteria

PsbP-like protein (sll1418) | cyanobacterial  in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS10 682)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from PsbP -like protein of Synechocystis sp. PCC 6803, UniProt: P73952

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

20.7 | 17 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803
Predicted reactivity

Anabaena variabilis, Arthrospira maxima, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes, Ostreococcus lucimarinus, Trichodesmium erythraeum,


Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot detection using anti-PsbP-like antibody

0.25 µg of chlorophyl a/lane from Synechocystis sp. PCC 6802 wilde type and PsbP-like deletion mutants were separated on 12.5 % SDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbP-like antibody (AS10 682, 1:2 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL detection reagent according the manufacturers instructions. Exposure time was 15 seconds.

Courtesy Dr. Sveshnikov

Additional information

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Algal protein extraction buffer

Secondary antibodies

Background

Background

PsbP-like protein (sll1418) is a cyanobacterial homologue of plant PsbP-like protein. It is localized in the thylakoid membrane and associated with photosystem II. Synonymes: Sll1418 protein

Product citations

Selected references Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Sveshnikov et al. (2007) The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II, Photosynth. Res. 93, 101-109.
Ishikawa et al. . (2005) Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803, Photosynth. Res. 84, 257-262.

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