Plant organelle/membrane isolation
Plant protein isolation
- Extraction of leaf proteins
- Diatom protein extraction
- Phenol protein extraction
- Protein extraction from grasses
- Ponceau membrane staining
- TCA acetone precipitation method
- Western blot protocol
- Western blot video tutorial
- Peptide neutralization/competition assay
- Simultaneous Western blot
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Dot blot
- ELISA
- Immunoprecipitation
- Immunoprecipitation/IgY
- Immunohistochemistry
- Anti-KLH antibody removal
- Yolk delipidation
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- IgY purification methods
- Protein purification using antibodies
- Elution of antibodies from affinity columns
Technical information > Antibody storageHow to store antibodies? General considerations
Total IgY fraction (IgY antibodies purified by precipitation from egg yolk)
Problem: affinity purified antibodies precipitated during storage in 4°C
Antibody solutions stored without preservatives are at the risk of being contaminated by bacterial growth, which is one of the most common reasons for protein inactivation.
• For larger volumes of affinity purified antibodies, filter-sterilize and aliquot the antibody sample to avoid repeated freezing and thawing.
• Thimerosal at 0.01% |
