Protocols
Plant organelle/membrane isolation
Purification
Plant organelle/membrane isolation
- Arabidopsis lumen extraction
- Arabidopsis thylakoid extraction
- BBY preparation
- Chlorophyll measurements
- Intact chloroplast isolation method
- Mitochondrial fraction
- Nuclear fraction
- Plasma membrane fraction
- PSII RC extraction for cryo-EM
- Thylakoid extraction
- Vacuol isolation
- Collection of articles
- Diatom protein extraction
- Extraction of leaf proteins
- Phenol protein extraction
- Ponceau membrane staining
- Protein extraction from grasses
- TCA acetone precipitation method
- Western blot protocol
- Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Western blot in denatured conditions (urea gel)
- Peptide neutralization/competition assay
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Simultaneous Western blot
- Anti-KLH antibody removal
- Dot blot
- ELISA
- Immunohistochemistry
- Immunoprecipitation
- Immunoprecipitation/IgY
- Meiotic staining
- Yolk delipidation
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- Elution of antibodies from affinity columns
- IgY purification methods
- Protein purification using antibodies
Protocols > Intact chloroplast isolation method
To evaluate purity of isolated chloroplasts different methods were applied, including: fluorescence microscopy, confocal imaging and subcellular fractionation assays. Purity of specific cellular fractions was confirmed using the following Agrisera antibodies: Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein Anti-RbcL | Rubisco large subunit, form I Anti-H3 | Histone H3 (rabbit antibody) (nuclear marker) Anti-MnSOD | Manganese superoxide dismutase Complete list of Agrisera plant cell compartment marker antibodies can be found here. |
