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Suggested Control Antibodies for Diatoms

AtpB | beta subunit of ATP synthase (10 µl) AS05 085-10 | clonality: polyclonal | host: rabbit 
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.
RbcL | Rubisco large subunit, form I and form II (10 µl)  AS03 037-10 | clonality: polyclonal | host: rabbit 
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.
PsbA | D1 protein of PSII, C-terminal (10 µl) AS05 084-10 | clonality: polyclonal | host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using a sensitive chemiluminescent detection system like AgriseraECLBright (extreme low femtogram range).

Protocol for protein extraction from diatoms. 

Below you will find antibodies with confirmed and predicted reactivity to Diatoms

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108 €

AS08 300  |  Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

292 €
Buy 2 items of this product for 218.00 €/items
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AS15 2992  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

381 €

AS03 030  |  Clonality: Polyclonal  |  Host: Chicken  |  Reactivity: [global antibody] for plant and bacterial F-type ATP synthases

292 €
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AS05 085  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga, animal and bacterial F-type ATP synthases

159 €

AS05 085-10  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga and bacterial F-type ATP synthases

292 €
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AS16 3976  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Brassica oleracea var. botrytis cv. ’Diadom’, [compartment marker] of mitochondrial inner membrane

89 €
By Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee
Hardcover: 204 pages Publisher: World Scientific Publishing Company (7 Jan. 2019)
Language: English
ISBN-10: 9813223103 ISBN-13: 978-9813223103

Product Number: AS19 4290Book ; Product Dimensions: 15.2 x 1.9 x 22.9 cm
292 €
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AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Scenedesmus sp.

374 €

AS10 935 | Clonality: Polyclonal | Host: Rabbit | Reactivity: diatoms

1408 €

AS05 070 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Dicots, monocots, algae

This discounted antibody set contains the following high titer antibodies: anti-Lhcb1, anti-RbcL, anti-PsaC, anti-PsbA, anti-AtpB, and respective protein standards for quantification. The set is suitable for Western blot using samples from a wide range of species making this product suitable for teaching labs or mutant screening. Before use on a specific species not listed on product information sheet, please contact us.

The set contains also high titer matching secondary antibody (AS09 602) and chemiluminescent detection reagent of mid picogram range

292 €
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AS17 4116 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Heterosigma akashiwo

292 €
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AS11 1789   |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.  acetabulum, A. thaliana, B. hypnoides (ulvophytes), G. theta, H. vulgare, Synechocystis sp., T. pseudonana, Z. mays

264 €

AS11 1789S  |  Protein standard for quantitation

Buy 2 positive controls/standards for the price of 1.
To get this discount please send your order to [email protected]
Follow this link to a list of discounted standards.<//span>

292 €
Buy 2 items of this product for 218.00 €/items
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AS13 2671 Clonality: Polyclonal Host: Chicken  Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae cellular [compartment marker] for plasma membrane

187 €
Buy 2 items of this product for 149.00 €/items
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AS08 371S  |  Protein standard for quantitation and positive control

357 €

AS08 282  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Diatoms

AS19 4303  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Phaeodactylum tricornutum

Interested to test this antibody? Please contact us.

AS19 4304   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Phaeodactylum tricornutum

Interested to test this antibody? Please contact us.

292 €
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AS19 4367 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Pheodactyllum tricornutum

292 €
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AS17 4115 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Cyclotella sp.Thalassiosira pseudonana

410 €

AS01 021A  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody]  for bacteria

292 €
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AS08 310  |  Clonality: Polyclonal  |  Host: Rabbit   |  Reactivity: A. thaliana, H. vulgare, C. reinhardtii, red alga Gracilaria gracilis, diatom Thalassiosira sp., P. tricornutum Bohlin, P. yunnanensis Dode, P. notoginseng, S. lycopersicum, S. tuberosum

315 €
AS18 4202 | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses
187 €

AS09 458S | Protein standard for quantitation and positive control

When you buy this product you get a 50% discount on other protein standards.
Follow this link to a list of discounted standards.
To get this discount please send your order to [email protected]

292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items

AS09 458 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

448 €

AS09 458-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

448 €

AS09 458-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

292 €
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AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. rapa subsp. chinensis, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, S. oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Z. mays

264 €

AS08 330S  |  Recombinant protein standard for quantitation

938 €

AS04 051 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants, algae, cyanobacteria

This discounted antibody set contains the following high titer, well published antibodies: anti-RbcL, anti-PsaC (PSI), anti-PsbA (PSII), and respective protein standards RbcL, PsaC, PsbA for quantification and determination of PSI/PSII ratio. The set is suitable for Western blot using samples from a wide range of species making this product suitable for teaching labs or mutant screening.

The set contains also high titer matching secondary antibody (AS09 602) and chemiluminescent detection reagent of mid picogram range

2193 €
SA000003  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes ten discounted, compartment marker antibodies of your choice.

Related products:

SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies

SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies

699 €
SA000001  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes three discounted, well published, compartment marker antibodies of your choice.

Related products:
SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies
SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies
1173 €

SA000002  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae

This set includes five discounted, compartment marker antibodies of your choice.

Related products:

SA000001 | Plant Cell Compartment Antibody Marker Set | 3 antibodies

SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies

314 €
Product no: AS18 PrimarySecondaryECL

This deal includes:
1. Primary antibody
2. Matching secondary antibody of high titer: Goat anti-rabbit, HRP conjugated (10 µl)
3. Two Agrisera chemiluminescence reagents, Agrisera ECL kit (Bright/SuperBright) trial pack
Low picogram (Agrisera Bright) and extreme low femtogram detection range (Agrisera SuperBright).
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items

AS07 257 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, O. sativa, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

448 €

AS07 257-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

448 €

AS07 257-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms

compartment marker of chloroplast stroma

292 €
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AS10 695 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, cyanobacteria

264 €

AS04 042S  |  Recombinant protein standard for quantitation


292 €
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AS10 939  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, algae, cyanobacteria, diatoms

387 €

AS04 042P | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, cyanobacteria

381 €

AS09 461 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Plants (monocots and dicots, conifers), moss: Physcomitrella patens, Chlamydomonas reinhardtii, Triticum aestivum, cyanobacteria

compartment marker of thylakoid membrane

292 €
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AS05 084A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms

compartment marker of thylakoid membrane

387 €

AS01 016  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody] for higher plants, algae, cyanobacteria and dinoflagellates

292 €
Buy 2 items of this product for 218.00 €/items
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AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

448 €

AS05 084A-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms

compartment marker of thylakoid membrane

427 €

AS05 084A-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms

compartment marker of thylakoid membrane

292 €
Buy 2 items of this product for 218.00 €/items
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AS11 1786  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants, alage, cyanobacteria

292 €
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AS13 2669 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants

274 €
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AS04 038 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, Physcomitrella patens, algae, cyanobacteria, diatoms

264 €

AS09 146S  |  Recombinant protein standard for quantitation and positive control

Buy 2 positive controls/standards for the price of 1.
To get this discount please send your order to [email protected]
Follow this link to a list of discounted standards.

292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items

AS06 146  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for A. thaliana, Anabaena 7120, D. brightwellii, H. vulgare, C.reinhardtii, C. zofingiensis, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, P. tricornutum, T. pratense, S. alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, T. guillardii, T. pseudonana, Triticale, U. prolifera, Z. mays

292 €
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AS15 2955 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  photosynthetic bacteria, archea, dinoflagellates


292 €
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AS03 037A Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms 

compartment marker  of chloroplast stroma in higher plants and cytoplasm in cyanobacteria

381 €

AS01 017  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody] for higher plants, algae, cyanobacteria  |  cellular [compartment marker] of plastid stroma

292 €
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AS03 037 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms 

compartment marker  of chloroplast stroma in higher plants and cytoplasm in cyanobacteria

448 €

AS03 037-ALP Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms  

compartment marker  of chloroplast stroma in higher plants and cytoplasm in cyanobacteria

448 €

AS03 037B Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, licHens,  algae, cyanobacteria, dinoflagellates, diatoms  

compartment marker  of chloroplast stroma in higher plants and cytoplasm in cyanobacteria

448 €
AS03 037-HRP Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms  |  cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria
187 €

AS01 017S  |  Protein standard for quantitation and positive control

When you buy this product you get a 50% discount on other protein standards.
Follow this link to a list of discounted standards.
To get this discount please send your order to [email protected]

AS19 4324  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Dunaliella tertiolecta

Interested to test this antibody? Please contact us.

292 €
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AS12 2111 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays, C. reinhardtii, S. lycopersicum

932 €

AS15 2994 | Reactivity: Rubisco protein

This set enables quantification of Rubisco using ELISA in samples from a range of various species. 

606 €
AS09 409 | Clonality: Polyclonal | Host: Rabbit

This set enables quantification of Rubisco using Western blot in samples from a range of various species  including dicots, monocots, algae, cyanobacteria. The set contains:
Most published anti-RbcL antibody (AS03 037)
Agrisera Protein Extraction Buffer (PEB)
High titer matching secondary antibody (AS09 602)
Chemiluminescent detection reagent of mid picogram range
187 €
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AS15 2873S |  protein standard for quantitation and positive control

292 €
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AS08 327  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Zea mays | Cellular [compartment marker] of Golgi in immunolocalization and of COP1 in western blot

292 €
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AS14 2775 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Vigna radiata

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