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Suggested Control Antibodies for diatoms

AtpB | beta subunit of ATP synthase (10 µl) AS05 085-10 | clonality: polyclonal | host: rabbit 
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.
RbcL | Rubisco large subunit, form I and form II (10 µl)  AS03 037-10 | clonality: polyclonal | host: rabbit 
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.
PsbA | D1 protein of PSII, C-terminal (10 µl) AS05 084-10 | clonality: polyclonal | host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using ECL Advance.
Note: TMA-6 from Lumigen gives similar results to ECL Advance.

Protocol for protein extraction from diatoms. 

Below you will find antibodies with confirmed and predicted reactivity to diatoms.


Agrisera Video Tutorial : western blot recommendations and trouble shooting News letter Protocols & technical information Protein extraction from diatoms
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100 €

AS08 300  |  Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

1324 €

SA000002  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants and algae


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SA000003- 2 | Plant Cell Compartment Antibody Marker Set | 10 antibodies

1310 €

AS05 070 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Dicots, monocots, algae

873 €

AS04 051 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants, algae, cyanobacteria

2647 €

SA000003 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants, algae

564 €

AS09 409 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Dicots, monocots, algae, cyanobacteria

310 €
AS15 3074-set  | Reactivity: higher plants and algae
272 €
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AS15 2992  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

355 €

AS03 030  |  Clonality: Polyclonal  |  Host: Chicken  |  Reactivity: [global antibody] for plant and bacterial F-type ATP synthases

148 €

AS05 085-10  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga and bacterial F-type ATP synthases

272 €
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AS05 085  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga, animal and bacterial F-type ATP synthases

272 €
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AS16 3976  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Brassica oleracea var. botrytis cv. ’Diadom

272 €
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AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

348 €

AS10 935 | Clonality: Polyclonal | Host: Rabbit | Reactivity: diatoms

272 €
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AS17 4116 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Heterosigma akashiwo

272 €
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AS11 1789   |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.  acetabulum, A. thaliana, B. hypnoides (ulvophytes), G. theta, H. vulgare, Synechocystis sp., T. pseudonana, Z. mays

245 €

AS11 1789S  |  Protein standard for quantitation

Buy 2 positive controls/standards for the price of 1.
To get this discount please send your order to orders@agrisera.com
Follow this link to a list of discounted standards.

272 €
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AS13 2671 Clonality: Polyclonal Host: Chicken  Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae cellular [compartment marker] for plasma membrane

174 €
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AS08 371S  |  Protein standard for quantitation and positive control

332 €

AS08 282  |  Clonality:Polyclonal  |  Host:Rabbit  |  Reactivity:Diatoms

272 €
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AS17 4115 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Cyclotella sp.Thalassiosira pseudonana

381 €

AS01 021A  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody]  for bacteria

407 €

AS08 310-100 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Ch. reinhardtii, red alga Gracilaria gracilis, diatom Thalassiosira sp. , P. tricornutum Bohlin, P. yunnanensis Dode, S. tuberosum

272 €
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AS08 310  |  Clonality: Polyclonal  |  Host: Rabbit   |  Reactivity: A. thaliana, H. vulgare, C. reinhardtii, red alga Gracilaria gracilis, diatom Thalassiosira sp., P. tricornutum Bohlin, P. yunnanensis Dode, S. lycopersicum, S. tuberosum

299 €
AS18 4202 | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses
174 €
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AS09 458S | Protein standard for quantitation and positive control

WHen you buy this product you get a 50% discount on other protein standards.
Follow this link to a list of discounted standards.
To get this discount please send your order to orders@agrisera.com

272 €
Buy 2 items of this product for 202 €/items
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AS09 458 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

417 €

AS09 458-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

417 €

AS09 458-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi