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HSP70 | Heat Shock Protein 70, plant ELISA kit

AS17 4128 | HSP70 (Plant) ELISA kit

HSP70 | Heat Shock Protein 70, plant ELISA kit in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS17 4128)
Qty: 
882

Data sheet Add review
Background | Sandwich ELISA (Enzyme-linked Immunosorbent Assay) is used to quantitate cytoplasmic plant HSP70 concentration in plant extracts including Medicago sativa, Linum usitatissimum, Zea mays. The plate is coated with anti-plant HSP70 capture antibody.

In most eucaryotes hsp70 genes exist as part of a multigene family and are found in most cellular compartments including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol. All HSP70 proteins, regardless of their location, bind particularly unfolded proteins. The universal ability of HSP70 proteins to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.  




This kit contains following reagents:

Item

Specifications (96 wells)

Storage

Micro ELISA Plate

1 plate

4°C

Recombinant Hsp70 (Alfalfa) Standard

2 vials/lyophilized

4°C

Sample / Standard dilution buffer *

1 vial/50 ml

4°C

5X Hsp70 Extraction Reagent *

1 vial/10 ml

4°C

10X Wash Buffer Concentrate *

1 vial/100 ml

4°C

Anti-HSP70 Biotinylated Antibody *

1 vial/150 µl

4°C  

Anti-HSP70 Biotinylated Antibody Diluent *

1 vial/13 ml

4°C

Streptavidin: HRP Concentrate

1 vial/ 50 µl

4°C  

Streptavidin: HRP Diluent

1 vial/13 ml

4°C

TMB Substrate

1 vial/13 ml

4°C

Stop Solution

1 vial/13 ml

4°C


* This reagent contains preservative: Kathon, 0.6 mg/ml.
Standard in this kit is not produced in baculovirus system.


Range:
1.563-100 ng/ml

Suggested standard curve points: 100 ng/ml, 50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.125 ng/ml, 0 ng/ml

Sensitivity:  0.18 ng/ml

Application: this kit can be applied for quantitative detection of cytoplasmic plant HSP70 protein in up to 40 samples.

Incubation time: 30 minutes

Storage: 4°C for 6 months. Unused wells should be resealed with desiccant in the foil pouch provided and stored at 4°C until the kits expiry date.

NOTE:  kit is for research purpose only. Not for human or animal disease diagnosis.



 
Typical standard curve for plant HSP70 ELISA kit. 

Typical standard curve for plant HSP70 ELISA kit
Manual in Pdf

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