HSP70 | Heat Shock Protein 70, plant ELISA kit

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Background | Sandwich ELISA (Enzyme-linked Immunosorbent Assay) is used to quantitate cytoplasmic plant HSP70 concentration in plant extracts including Medicago sativa, Linum usitatissimum, Zea mays. The plate is coated with anti-plant HSP70 capture antibody.

In most eucaryotes hsp70 genes exist as part of a multigene family and are found in most cellular compartments including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol. All HSP70 proteins, regardless of their location, bind particularly unfolded proteins. The universal ability of HSP70 proteins to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.  

This kit contains following reagents:

Item	Specifications (96 wells)	Storage
Micro ELISA Plate	1 plate	4°C
Recombinant Hsp70 (Alfalfa) Standard	2 vials/lyophilized	4°C
Sample / Standard dilution buffer *	1 vial/50 ml	4°C
5X Hsp70 Extraction Reagent *	1 vial/10 ml	4°C
10X Wash Buffer Concentrate *	1 vial/100 ml	4°C
Anti-HSP70 Biotinylated Antibody *	1 vial/150 µl	4°C
Anti-HSP70 Biotinylated Antibody Diluent *	1 vial/13 ml	4°C
Streptavidin: HRP Concentrate	1 vial/ 50 µl	4°C
Streptavidin: HRP Diluent	1 vial/13 ml	4°C
TMB Substrate	1 vial/13 ml	4°C
Stop Solution	1 vial/13 ml	4°C

* This reagent contains preservative: Kathon, 0.6 mg/ml. Standard in this kit is not produced in baculovirus system.