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Western blot using anti-BAK1 antibodies

In the example above, the same antibody was used (Anti-BAK1, AS12 1858) but the proteins were extracted with two different buffers.
Minor adjustments in the buffer components resulted in the improved detection of the target protein - from NO band to a band of correct MW. 

If you are interested in more tricks to optimize your western blot, you are welcome to contact us or request a copy of the Agrisera Western Blot Guide.
2019-10-07 @ 07:59:22