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Western blot using anti-BAK1 antibodies

In this case, the same antibody was used (Anti-BAK1, AS12 1858) but proteins were extracted with two different buffers.
Minor adjustments in the buffer components resulted in the improved detection of a target protein - from NO band to a band of correct MW. 

If you are interested in more trick to optimize your western blot, you are welcome to contact us or request Agrisera Western Blot Guide
2019-10-07 @ 07:59:22