Western blot using anti-BAK1 antibodies

In the example above, the same antibody was used (Anti-BAK1, AS12 1858) but the proteins were extracted with two different buffers.
Minor adjustments in the buffer components resulted in the improved detection of the target protein - from NO band to a band of correct MW. 

If you are interested in more tricks to optimize your western blot, you are welcome to contact us or request a copy of the Agrisera Western Blot Guide.
 Agrisera blog

Agrisera blog