Can the same protocol can be applied to all antibodies?

No, this is NOT the case.

Each protein-antibody pair may require slightly modified binding and detection conditions. The variations may for example be a membrane with another pore size and physical properties (nitrocellulose or PVDF) as well as different blockers, incubation times and primary antibody dilutions. Some antibodies may work better in TBS-T buffer, others with PBS-T.

These issues are disussed in Agrisera Western blot guide, which we recommend you to keep in your lab!

Western Blot Guide   Get your free copy of the Agrisera Western Blot Guide Click here to get your copy!