Loading controls are used to normalize the levels of proteins detected using Western blotting, and will:

  • confirm that there is an equal loading across the gel
  • confirm that the protein transfer is even throughout the whole gel
  • allow qantification
  • can be requested by referees of scientific publications


Proteins of high levels of constitutive expression are usually recommended as loading controls. However, the choice of the appropriate house-keeping protein depends on the experiment in question, and thus needs to be confirmed. House-keeping proteins cannot be used for normalization if there is a suspicion that the experimental conditions are affecting their expression.

Agrisera's Collection of Antibodies to Loading Controls can be found here. Until end of 2020 they are offered at 15 % off. 

 
 

Agrisera Loading Control Antibodies


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