Quantify GlnA enzyme in samples from wide range of organisms

Glutamine synthetase GlnA is the key enzyme in the incorporation of mineral nitrogen into glutamine. The activity of this enzyme is controlled by adenylarion under conditions of abundant glutamine.

Agrisera Global GlnA antibodies are reactive to this enzyme from the following species, as confirmed experimentally: Deinococcus radioduransm Synechococcus sp. strain PCC 7942, Synechocystis sp. strain PCC 6803, Trichodesmium IMS

Beyond this, reactivity is predicted to the following species, based on sequence homology: Alpha, beta, gamma proteobacteria, Arthropsira sp. PCC 8005, Crenarchaeotes, Enterobacteria, Escherichia coli, Euryarchaeotes, Thermotogales

Confirmed and predicted reactivity is explained further here.

By using GlnA Global antibodies, combined with GlnA protein standard it is possible to quantify GlnA using quantitative Western blot, described here, and in this video tutorial.

Until end of March 2023, both products are offered with a 20% discount.

Quantify GlnA enzyme in a wide range of samples

3 µg of total protein from Trichodesmium IMS 101 extracted with Agrisera Protein Extration Buffer (AS08 300) (1-8) and GlnA protein standard 0.3, 0.15, 0.07 pmol (9-11) were used to visualise and quantite GlnA.

Quantify GlnA enzyme in samples from wide range of organisms

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