Protein Extraction Buffer (PEB) is an extraction buffer for the disruption and solubilization of total protein from plant tissues and algal cells. The buffer contains the anionic detergent LDS, which does not form crystals while stored at 4-10°C, and is quickly ready to use. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure), depending on the starting material, e.g. its biological stage and homogenization method used (bead beater vs. sonication). For processing of multiple samples, simultaneous extraction using for example a bead beater is recommended, for consistency of extraction. Provided as 5 x 2 ml (4x stock), Agrisera's PEB buffer allows up to 75 isolations of plant material (using 500 μl 1x PEB for 100 mg fresh weight), or 190 isolations of algal material (using 200 μl 1x PEB for cell amounts corresponding to 4-10 μg total chlorophyll). To product page: PEB (4x) | protein extraction buffer | Chosen references: • Altuntas et al. (2020). Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144(1):35-48. doi: 10.1007/s11120-020-00727-w. • Pérez-López et al. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784. • Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1â??20. (sea-ice diatom) • Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80. |
Latest
Meet with Agrisera at Chlamy 20252025-09-25 Two conferences supported by Agrisera this week
2025-08-22 Interview with Prof. Wolfgang Schröder
2025-08-21 Antibodies for research validation of genetically modified plants
2025-08-18 Agrisera supported Canadian Society of Plant Biologists 2025 Annual Meeting
2025-08-13 Interview with Dr. Pushan Bag
2025-08-11 Light-induced structural changes in thylakoid membranes, revisited in the recent publication
2025-07-29 Agrisera supported 20th Congress on Molecular Plant-Microbe Interactions
2025-07-25 Interview with Prof. Ru Zhang
2025-07-23 What links Agrisera antibodies to photosynthetic proteins and restoring anabolism of myocardial injury
2025-07-17
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