Question: If something goes wrong with the development of a Western blot with ECL, or if I have too high background or too low signal, can I redo the reaction on the same blot? Answer: Yes! Within the same day. Simply wash the membrane with the appropriate washing buffer, used in your protocol, 1-2 times for 5-10 minutes, and apply another one of the ECL reagents from the Agrisera ECL reagent set. If there is too much background, and a very strong band for the target protein, apply Agrisera ECL Bright. If a target protein band is not detected, apply Agrisera ECL SuperBright. If you want to learn more about how to improve your Western blot, click here. | Blot A shows an abundant photosynthetic protein, PsbA, visualized with too sensitive of a reagent, which was washed away, and the membrane was subsequently developed with less sensitive Agrisera ECL Bright (Blot B). |
Latest
Agrisera Western blot workshop part II with Yao-Hong Biotechnology for researchers in Taiwan2024-11-08 Visit of researchers from KBC to Agrisera facility in Umeĺ
2024-11-07 Agrisera at KBC Days 2024
2024-11-06 Agrisera supported 50th Midwest Southeast Photosynthesis Conference
2024-11-04 Visualize plant cell wall components with monoclonal antibodies
2024-11-01 Agrisera at the local events this week
2024-10-25 Mitochondria in focus, a set of new antibodies
2024-10-24 Agrisera supported 6th ZMBP Summer Academy
2024-10-18 Agrisera Western blot workshop for Michigan State University
2024-10-11 Agrisera Western blot workshop with Yao-Hong Biotechnology for researchers in Taiwan
2024-10-01
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