Improved method of isolation of intact chloroplasts, validated with Agrisera antibodies

A group of researchers from India, published an interesting article recently: "An improved method for isolating intact chloroplasts from different plant species".

Developed protocol was optimized for several species, including Nicotiana benthamiana, Arabidopsis thaliana, Oryza sativa, Solanum lycopersicum and secondary metabolite-rich plants: Stevia rebaudiana, Camellia sinensis, and Sambucus javanica.

To evaluate purity of isolated chloroplasts different methods were applied, including: fluorescence microscopy, confocal imaging and subcellular fractionation assays. Purity of specific cellular fractions was confirmed using the following Agrisera antibodies:

Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein
Anti-RbcL | Rubisco large subunit, form I
Anti-H3 | Histone H3 (rabbit antibody) (nuclear marker)
Anti-MnSOD | Manganese superoxide dismutase

Complete list of Agrisera plant cell compartment marker antibodies can be found here.

Plant chloroplast

Improved method of isolation of intact chloroplasts, validated with Agrisera antibodies

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