New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors

mNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm, an emission maximum at 517 nm, and is therefore compatible with most GFP filter sets.

mNeonGreen is 3x brighter, more stable, and does not bleach as fast as GFP. This makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has a MW of 26.6 kDa.

The peptide used to elicit the Agrisera mNeonGreen antibody is conserved in the following expression vectors:

dCas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM020]
Cas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM006]
mNeonGreen4-tDeg [Cloning vector pminiCMV-mNeonGreen4-tDeg]
ER-localized mNEONGREEN [Binary vector pKT-NM-erNEON]
mNeonGreen-3xFLAG [Cloning vector pLM160-mNeonGreen]
mNeonGreen-ty1 [Cloning vector pSAG1-mNeonGreen_TUB1-dTomato]
mNeonGreen, partial [Binary vector pRATIO2131]

Agrisera's mNeonGreen antibody, product number AS21 4525, recognizes mNeonGreen tags expressed in both plant and algal samples.

Experimental details for this Western blot can be found here.

New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors

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