Loading control antibodies: a way to ensure that the obtained Western blot results are accurate

Loading control antibodies are used to assess if the protein samples have been loaded equally across the gel, as well as transferred to the membrane. Such controls need to be ubiquitously and constitutively expressed proteins. Commonly used loading controls are the following proteins: actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and tubulin. 

Actin and tubulin are highly conserved proteins, which play essential roles in the plant cytoskeleton. Actin is involved in cellular processes, such as cell division, cell share maintenance and intracellular transport. 
Tubulin is a component of micotubules, which are critical for cell division, intracellular transport and maintaining cell structure. 
 
A protein that is chosen to be used as a loading control should remain stable across the applied experimental conditions. Please note that in certain stresses in plants, actin levels may change. 

Take an advantage of Agrisera's ongoing promotion for plant developmental biology antibodies, which are offered at a 20% discount until end of September, and use promotional code Dev20. 

• Polyclonal antibodies to plant actin (most published) AS13 2640 with a wide species range of di and monocotyl plants.
• Monoclonal/recombinant antibodies to plant actin (most published) AS21 4615 with a wide species range of di and monocotyl plants. 
• Polyclonal antibodies to: Tubulin alpha chain | Tubulin beta chain | Tubulin gamma chain  
• Monoclonal antibodies to: Tubulin alpha chain | Tubulin beta chain | Tubulin gamma chain
  
  
  Available for immunolocalization as well as Western blot techniques.