Agrisera blog

Our warmest congratulations to Maria Belen Velasquez from Rosario National University in Argentina, who received the Agrisera Best Poster Prize for her poster: "The Secretome of the green alga Scenedesmus sp." during this year's meeting of Argentinian Society fo Biochemistry and Molecular Biology, SAIB.

Awaiting your Agrisera product choice!

Agrisera antibodies have a broad reactivity including diatoms, algae, higher plants. For an overview, please click here. 

KBC Days is a yearly event at the Chemistry and Biology House at Umeå University. The programme this year included inspiring presentations by new faculty members and award recipients, and 11 poster presentations from midterm PhD students. Guided tours to present the infrastructures/platforms were also offered.

Thank you for this fruitful and interesting event!

During the recent 45th Annual Midwest/Southeast Photosynthesis Conference in Indiana, the Agrisera Best Talk Prize was awarded to Kelsey Dahlgren from the University of Colorado, Boulder. Kelsey works in Dr Jeffrey Cameron’s lab and gave an outstanding presentation entitled, "Development of a proximity-based proteomics technique using APEX2 to determine the thylakoid lumen proteome of Synechococcus sp. 7002." She is using both mass spectrometry and immunoblotting to characterize the lumenal proteome. 

Our warmest congratulations Kelsey. We are waiting your free antibody choice!

If no signal is detected in a total cell extract, it is worth fractionating, to obtain specific chloroplast, mitochondria or nuclei enriched fractions. This procedure will concentrate a target protein and lead to the detection of a desired band (for non-cytoplasmic proteins). This is shown in the example above - from no band in a total cell extract, to a specific band in photosystem I fraction.
This approach is especially important when working with proteins of low expression. Fractionation combined with using a specific organ, e.g. the root tip, or a leaf at a certain developmental stage, will greatly contribute to the successful identification of your protein of interest. 

Examples of protocols to fractionate can be found here.
For Agrisera Western Blot Guide and video tutorials, check here.
In case of any questions, you are always welcome to contact Agrisera Technical Support. 

Two primary antibodies incubated on the blot at the same time - save time in the lab

Thylakoid membranes (10 µg of total chlorophyll) extracted freshly from Hordeum vulgare leaves with 100 mM HEPES-KOH (pH 7.5), 0.3 M sorbitol, 2 mM EDTA, and 1mM MgCl2 and denatured with a Laemmli buffer at 80°C for 5 min were separated on 12% SDS-PAGE and blotted 1 h to nitrocellulose (pore size of 0.2 um), using semi-dry transfer. Blot was blocked with 4% milk for 2 h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:3000 (PC and PetA, simultaneous western blot detection for both antibodies at the same time) for 1 h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25000 in for 1 h/RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to manufactures recommendations. Exposure time was 30 seconds. Simultaneous western blot detection can be applied if MW of detected proteins differs in min. 20 kDa.

Courtesy Dr. Anja Liszkay, CNRS, France

Between the 9th and the 12th of September, Agrisera participated in the 9th biennial Polish Society of Experimental Biology Conference hosted by the Faculty of Biology and Environmental Protection, at the Nicolaus Copernicus University in Toruń, Poland. The theme of the Conference was "New trends in plant reproduction and growth regulation". The Best Poster Prize was funded by Agrisera and awarded to Dawid Kubiak from the group of prof. Janusz Niedojadło (bottom left on the picture) from the Department of Cellular and Molecular Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Toruń. The title of the poster was "Epigenetic and epitranscriptomic regulation of gene expression". 

On the third day of the conference, Dr. Joanna Porankiewicz Asplund from Agrisera, held a workshop named "Tips and tricks of antibody production and western blot - how to obtain good results?". Thank you to all who participated!
The winner of the Agrisera Kahoot interactive quiz, who received a secondary antibody ECL set, was: Aleksandra Rosińska (top left picture) from Dr. Krystyna Oracz lab. The set contains two chemiluminescent detection reagents (mid picogram and extreme low femtogram range) and an Agrisera goat anti-rabbit HRP-conjugated secondary antibody of high titer (min. 1: 25 000 1h/RT). 

Conference participants could test their weight measuring skills in an Agrisera quiz, conducted during each day of a conference. The winner, who correctly determined the weight of our secondary antibody ECL set, was Magdalena Czołpińska from UAM in Poznań. 

Thank you to everyone who came to the Agrisera table, to talk antibodies, share your experience with western blot, tell us about your work or participate in the quiz.

"On the role of phosphorylation of serine residues in aggregation mechanism of amyloid β-peptide"

We are happy to let you know, that Agrisera Team has new members on board. 

Annika
Annika Nordstrand holds an MSc in Molecular Biology and a PhD in Oncology from Umeå University. She has over ten years of laboratory experience from research mainly targeted at understanding the importance of the tumor microenvironment in prostate cancer bone metastases. Her work has involved both development of new in vitro models and treatment experiments in different in vivo models, as well as establishment of cell cultures directly from patient tumors. She has experience in several techniques involving antibodies, such as western blot, ELISA, and immunohistochemistry. At Agrisera she is going to work with sales support. In her spare time she loves to do creative things and spend time with family and friends.

Caroline
Caroline Zetterström has a Master of Science in Biotechnology and a PhD in Chemistry from Umeå University. During her PhD the research focused on development of alternatives to antibiotics in gram-negative bacteria by inhibiting their virulence systems, their ability to cause disease. She has a long experience of lab work, with a variety of chemical and microbiological techniques e.g. western blot, cell culturing, organic synthesis, cloning, protein expression and purification. She has done click chemistry assays on live bacteria in a microfluid plate underneath the spinning disc confocal microscope. At Agrisera she is going to work with antibody purification, QC and product development. 
In her free time she likes to be with her family out in the nature and sew clothing for herself.

Edith
Edith Kalén holds a degree in engineering science within the field of biotechnology, obtained at Umeå University. She did her master’s degree in plant physiology at Umeå Plant Science Centre (UPSC),  with prof. Stefan Jansson and Prof. Laszlo Bako. She obtained practical and theoretical knowledge in areas such as microbiology, functional genomics, gene expression and genetics. Edith also has a background in scientific writing and graphic design, and is involved in preparation of Agrisera marketing materials and conference attendance. You may meet her soon as she will be attending events such as the 30th International Conference on Arabidopsis Research (ICAR2019) in Wuhan, China, as well as Plant Biology (PB19) in San José, US.
In her spare time, Edith enjoys spending time in nature and attending various cultural events.

• There are few ways to easy receive an overwiev on coming meetings
• Using a simple tool, plant event can be easily included into the calendar
• A mini calendar widget can be loaded to a conference/society website, to obtain a widget contact: [email protected]
• There is an option to subscribe to the calendar
• Simple search can be done using specific key words

• recommended dilution and applications (Product information tab)
• experimentally confirmed species reactivity and predicted reactivity, which is deduced from antigen sequence conservation (Reactivity)
• images and detailed descriptions (Application examples)
• specific details about the product (Additional information)
• a list of relevant products (Related products)
• a comprehensive list of publications in which antibody was applied (Product citations)