Agrisera blog

• FNR1 | Ferredoxin NADP Reductase, isoprotein 1 (leaf)
• FNR2 | Ferredoxin NADP Reductase, isoprotein 2 (leaf)
• FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf)
• FNR | Ferredoxin NADP Reductase (root)
• Fd1 | Ferredoxin 1 (chloroplastic)
• Fd2 | Ferredoxin 2 (chloroplastic)
• Fd3 | Ferredoxin 3
• Fd1, Fd2, Fd3, Fd4 | Ferredoxin 1,2,3,4
• FdC1 | Ferredoxinx-C1

(Agrisera), AS04 051

Western Blot Rubisco quantitation kit

(Agrisera), AS09 409

RbcL | Rubisco large subunit, form I, AS03 037, not conjugated, AS03 037-HRP, conjugated to HRP or AS03 037-APL, conjugated to ALP with a very wide reactivity range: di and monocots, trees, mosses, ferns, alage. Antibody has a matching protein standard for Rubisco quantification, using quantitative western blot technique. 

RbcL II | Rubisco large subunit, form II AS15 2955, with reactivity to: photosynthetic bacteria, archea, dinoflagellates. Antibody can be combined with a respective protein standard for quantification, using quantitative western blot technique. 

Through months July to September this collection is offered with a 30 % discount with a discount code: Rubisco30

Agrisera antibody sets will allow quantification of major photosynthetic proteins in important photosynthetic organisms:
cyanobacteria, 
diatoms, 
green algae such as Nannochloropsis and Chlamydomonas reinhardii

Rubisco quantitation kit
The kit contains: anti-RbcL antibody, Rubisco protein standard, high titer secondary antibody and chemiluminescent detection reagent for quantification of Rubisco using quantitative Western blot technique. 

Photosynthesis Tool Kit - quantitation
The kit contains: anti-RbcL antibody, Anti-PsaC antibody, Anti-PsbA antibody, respective protein standards, high titer secondary antibody and chemiluminescent detection reagent for quantification of Rubisco, PSI and PSII using quantitative Western blot technique. 

Oxidative Stress Antibody Set
contains 5 antibodies of your choice with reactivity to plant and algal species:
APX, Cat, Cu/ZnSOD, FeSOD, Gamma-ECS, GR, GS,  HRP, MnSOD, RBOHD.

For species not listed on product info sheet, please inquire. 

If algal cellular fraction purity needs to be confirmed, Agrisera offers a set of Algal Compartment Marker Antibodies. These antibodies can be matched to your specific experimental requirements.

Antibodies to cyanobacteria, diatoms, green algae such as Nannochloropsis and Chlamydomonas reinhardii are offered with a 15 % discount until end of June. Use discount code: Ocean15

Pictures of various species:
Diatoms, cyanobacteria -  courtesy of Dr. Douglas Campbell
Nannochloropsis oceanica strain CCMP1779 - Christopher W. Gee, Ph.D. Niyogi Lab
Spirogyra, C. tumidulum and C.astroideum - Dr. Natalia Voronkina

The 8th of June is the international World Oceans Day. To celebrate, Agrisera is offering a 15% discount on all antibodies for important photosynthetic organisms of the oceans. These include cyanobacteria, diatoms, green algae such as Nannochloropsis and Chlamydomonas reinhardii, dinoflagellates that power coral photosynthesis, macrophyte algae such as porphyra, and marine plants, such as the sea grasses that shape coastal ecosystems. Agrisera's Global Antibodies product range is suitable for the detection of various proteins from these species. If your species of interest is not listed in the product information sheets, please inquire. 

If algal cellular fraction purity needs to be confirmed, Agrisera offers a set of Algal Compartment Marker Antibodies. These antibodies can be matched to your specific experimental requirements.

Many of the antibodies in these collections have been used in diverse research projects for many years and have quite an extensive publication record.

If you cannot find a specific antibody needed for your research in the Agrisera Catalog, you are welcome to contact us. We are always looking for ideas to develop new antibodies or can tailor custom antibody production to your specific project.

Pictures of various species:
Diatoms and cyanobacteria -  courtesy of Dr. Douglas Campbell
Nannochloropsis oceanica strain CCMP1779 - Christopher W. Gee, Ph.D. Niyogi Lab
Spirogyra, C. tumidulum and C.astroideum - Dr. Natalia Voronkina

We have the pleasure to announce that as of the 7^th of May 2020, Agrisera has a new owner – Olink Proteomics AB.

Agrisera and Olink have cooperated for a long time, and we are now taking the great relationship that we have established over the years to the next level. Olink Proteomics is a Swedish company that provides innovative solutions for protein biomarker discovery and development with rapid, high-throughput immunoassay analysis, exceptional data quality, and minimal sample consumption.

Agrisera will remain in its current form, with me still in the position as CEO and with no major changes to the organization as a whole. The people you have been in contact with will continue to be there for you.

We will maintain our online shop with focus on antibodies for plant science, as well as grow and develop our product catalog in the true spirit of Agrisera.

Moving forward, we will likewise keep the same high demands on all portions of our production chain to continue providing you with the same high-quality products and services that Agrisera stand for.

We are sure that Agrisera, as well as our partners and customers, will benefit by the strength of our new owner. We value our relationship with you greatly and look forward to continuing to grow together with you while becoming an even better supplier for the antibodies and reagents you need.

We understand that you may have further questions regarding this development, and you are very welcome to contact me.

Best regards,

Erika Gelfgren, CEO, Agrisera AB

[email protected]

Agrisera offers a range of universal reagents, required in every laboratory using Western blot, ELISA or immunolocalization techniques. 

Secondary antibodies
Over 2000 validated, high-quality antibodies for immunoglobulins from different species:

Anti-Bovine, Anti-Cat, Anti-Chicken, Anti-Dog, Anti-Donkey, Anti-Goat, Anti-Guinea pig, Anti-Hamster, Anti-Human, Anti-Llama, Anti-Mouse, Anti-Rabbit, Anti-Rat, Anti-Sheep.

These are offered both conjugated and unconjugated, for luminescent, fluorescent and chromogenic detection in a wide range of applications, such as ELISA, Immunolocalization studies and Western blot.

Detection reagents
Agrisera chemiluminescent detection reagents are developed using unique chemistry with low pico to mid femtogram and extreme low femto detection levels. These reagents also offer increased stability, 24 month shelf life and superior lot-to-lot consistency at a very competitive price.

Agrisera BCIP/NBT ALP Substrate is designed to localize ALP labeled probes on Western, Northern, Southern and Dot blots, while Agrisera BCIP/NBT Plus ALP Substrate is designed for immunohistochemical, in situ hybridization and blotting techniques. These chromogenic reagents are ready-to-use, single component, give no to little background and contain non-toxic stabilizers.

Blocking reagents
For most immunoassays like IHC, ELISA or Western blot, blocking is a crucial step to perform. It blocks spaces that are not occupied by immobilized proteins, which prevents non-specific binding of the antibodies used in the assay.

Agrisera offers the following groups of normal serum blockers: Bovine Serum/IgG, Cat Serum/IgG, Chicken Serum/Ig Fraction, Dog Serum/IgG, Donkey Serum/IgG, Duck Serum/IgG, Feline Serum/IgG, Goat Serum/IgG, Guinea pig Serum/IgG, Hamster Serum/IgG, Horse Serum/IgG, Human Serum/IgG, Llama Serum/IgG, Mouse Serum/IgG, Pig Serum/IgG, Rabbit Serum/IgG, Rat Serum/IgG and Sheep Serum/IgG.

All Agrisera reagents are offered with: 

• Quick and easy delivery worldwide
• Bulk volumes available on request
• Skilled technical support by email and live chat

Our warmest congratulations to PhD student Shvaita Madhuri in professor Kroth's group at University of Konstanz, who was awarded the Agrisera Best Poster Prize during the 18th Scientific Conference of the Phycology Section with the German Botanical Society, in Kall-Steinfeld in Germany, March 8th-11th. The title of the awarded poster was: "Are areochromes involved in regulating diel biological rhythm of Phaeodactylum tricornutum".

In the above picture, left to right: the president of Phycology Section of DGB, Prof. Dr. Maria Mittag, the awardee, Shavaita Madhuri, and the secretary Dr. Andreas Holzinger, from University of Innsbruck, Austria.

Awaiting your free antibody choice!
Agrisera collection of antibodies to diatom proteins can be found here. 

• -20°C or -80°C
• +4°C with preservatives like azide (0.02%) or merthiolate
• -20°C with glycerol at a final concentration of 10% or 50% (for affinity purified antibodies)
• -20°C with BSA at final concentration of 0.05-0.5% (for affinity purified antibodies)

More information on what to consider when storing different kinds of antibodies can be found here. 

A known problem is that affinity purified antibodies can precipitate when stored at 4°C. However, such antibodies may still maintain their activity towards a target protein. 

For catalog antibodies, we highly recommend checking the product information sheet, which is always included with the product, and contains proper information about storage conditions. If you have further questions, you can request additional information by contacting us. 

Good luck with your research!

Agrisera participated in the Epichrome 2020 meeting taking place at Umeå University, Sweden, between 27.02-28.02. 

Our warmest congratulations to Dr. Eva Brinkman from Pelechano Lab at SciLifeLab, who was awarded the Agrisera Best Poster Prize at this meeting. The poster title was: "Genetic wiring maps of co-acting transcriptional factors in single cells".

Awaiting your free antibody choice!

Agrisera collection for epigenetic research can be found here.

(Agrisera), AS04 051

Western Blot Rubisco quantitation kit

(Agrisera), AS09 409

Beside primary antibodies and quantification standards, the kits also contain matching secondary antibodies of high titer, min. 1> 25 000 1h/RT (AS09 602) and suitable chemiluminescent detection reagent, AgriseraBright in mid picogram range. 

Example

Figure 1: Immunoblot of samples of Synechococcus elongatus PCC 7942 (lanes 1-4) and Anabaena sp. PCC 7120 (lanes 5-8). Molecular weight markers (MagicMark XP, Invitrogen) are in lane 9. Recombinant PsbA protein standards are loaded in lanes 10-12 at 0.05 pmoles, 0.15 pmoles and 0.45 pmoles.

The blot was quantitated with QuantityOne software and the standard curve is shown in Figure 2.

Figure 2: Standard curve for the blot in Figure 1. Picomoles of quantitated standard is plotted on the X-axis versus the adjusted signal volume obtained on the Y-axis.

Quantitative Western Blot method is described here. You are also welcome to watch a short video tutorial. 

Western Photosynthesis Conference, 2.01-5.01, Bodega Bay Marine Laboratory (BBML), Bodega Bay, USA 
Umeaå Center for Microbial Research Day, 23.01, Umeå University, Umeå, Sweden
33rd Conference Molecular Biology of Plants, 11.02-14.02, Dabringhausen, Germany
Solar Energy to Biomass, 11.02-14.02, Hotel Vila Galé Porto, Porto, Portugal
CSPB/SCBV Western Regional Meeting, 18.02-19.02, Canmore, Canada
Epichrom 2020, 27.02-28.02, Umeå University, Umeå, Sweden
Meeting of the Spanish Society of Autophagy (SEFAGIA), 4.03-6.03, Cáceres, Spain
18th Scientific Conference of the Phycology Section, 8.03-11.03, Kall-Steinfeld, Germany
ASPB Annual Plant Biology Meeting - (PlantBio20), 25.07-29.07, Wardman Park Marriott, Washington, DC, USA, virtual worldwide summit
11th SPPS phd student conference, 2.09-4.09, Turku, Finland, virtual event
PostDoc Day at University of Geneva, 17.09, Geneva, Switerland, virtual event
BioLOGIES: Bionspiration, Inventions and Breakthrough technologies, 22th-24th September, Poznan, Poland, virtual event
46th Annual Midwest/Southeast Photosynthesis Meeting, 23.10-24.10, Michigan State University, USA, virtual event
1st Brazilian Symposium on Photosynthesis, 27.10-30.10, Brazil, virtual event
KBC Days 2020, 3.11-4.11, Umeå, Sweden
CSPB/SCBV 2020, 7.11, Canada, virtual event
XV Meeting of Plant Molecular Biology, 26.11-27.11, Malaga, Spain, virtual event

Understanding Plant Responses to Climate Changes: Redox based strategies, postponed, Baeza, Spain
Eastern Region Photosynthesis Conference, 24.04-26.04, Woods Hole, USA, cancelled, webinar instead
Austrian Plant Biology Society meeting, 21.05-23.05, Seitenstetten, Austria, postponed to 2021
19th International Conference on the Cell and Molecular Biology of Chlamydomonas, 24.05-29.05, Les îles Paul Ricard, Six-Fours-les-Plages, France, postponed to 2021
XV Plant Molecular Biology Meeting, 3th-5th of June, Málaga, Spain, postponed
Plant Biology Europe, 29.06-2.07, Centro Congressi Lingotto, Torino, Italy, postponed to 2021
PCS2020 Plant Calcium Signaling Conference, 6.07-8.07, Milano, Italy, postponed to 2021
8th Plant Nitric Oxide International Meeting, 8.07-10.07, Szeged, Hungary, postoponed to 2021
4th International Plant Proteomics Organization Conference, 13.09-16.09, Toronto, Canada, postponed to 2021
6th DNA POlymerases Meeting, 20.09-23.09, Stockhom, Sweden, postponed to 2021
RAFV2020, 5-8.10, Santa Fe, Argentina, postponed to 2021

Due to Covid19 pandemic many conferences are cancelled, postponed or moved to 2021 or 2022.
This information is regularly updated in The Global Plant Events Calendar. 

Warmest congratulations to Anis Meschichi, doctoral student at The Swedish University of Agriculturala Sciences (SLU), who received the Agrisera Best Poster Prize for his poster: "CHARACTERIZING CHROMATIN MOBILITY AND HISTONE DYNAMICS DURING DNA DAMAGE RESPONSES" during the Impact of Chromatin Domains on Plant Phenotypes meeting taking place in Madrid between 9th and 11th of December.

Awaiting your antibody choice!

Jan Karlsson received the UPSC Agrisera Prize 2019 to acknowledge his ceaseless technical support for the UPSC community at UPSC (Umeå Plant Science Centre). The announcement was done during the Christmas lunch, and the motivation from one of the nominations was as follows: "He is always there for the whole institute to help to solve all kind of technical, computational, mechanical or physical strength-requiring problems. He has spent the night at work to make sure that the backup power system will not fail when there was a planned overnight power interruption. He did a tremendous amount of work with Thomas Hiltonen for the moving of Plant Physiology Department during the moving out from old labs, the reconstruction and moving into the new lab. He supervises all growth chambers, shakers and cabinets, and he is the one, who we hope to find in his office when we need a solution for a challenging technical issue. People can always ask for his advice or help, independently from which department they belong to." 

This year the prize was presented by Joanna Porankiewicz-Asplund from Agrisera and by the chair of the UPSC Board, Catherine Bellini. From left to right: Joanna Porankiewicz-Asplund (Agrisera), Catherine Bellini (Chair of the UPSC board), Jan Karlsson. Photo: Anne Honsel.

Our warmest congratulations to Maria Belen Velasquez from Rosario National University in Argentina, who received the Agrisera Best Poster Prize for her poster: "The Secretome of the green alga Scenedesmus sp." during this year's meeting of Argentinian Society fo Biochemistry and Molecular Biology, SAIB.

Awaiting your Agrisera product choice!

Agrisera antibodies have a broad reactivity including diatoms, algae, higher plants. For an overview, please click here.